monoclonal anti abca1 antibody  (Cell Signaling Technology Inc)

Journal: iScience

Article Title: Integrating proteomics and metabolomics to reveal MAT2A for metabolic reprogramming in non-small cell lung cancer cells

doi: 10.1016/j.isci.2026.117336

Figure Lengend Snippet:

Article Snippet: Rabbit monoclonal anti-ABCA1 , ABclonal , Cat# A22125.

Techniques: Immunohistochemistry, Staining, Western Blot, Recombinant, Modification, Sequencing, Solvent, Transfection, CCK-8 Assay, Gene Expression, Negative Control, Software

Journal: Frontiers in Immunology

Article Title: Ex vivo expanded human regulatory T cells promote cholesterol efflux and PON1 expression in oxLDL-exposed macrophages via gap junction-mediated cAMP transfer

doi: 10.3389/fimmu.2025.1662925

Figure Lengend Snippet: Treg exp increase ABCA1 expression by transferring cAMP into M IL4 . (A) Changes in the expression of ABCA1 and ABCG1 transcripts in M IL4 co-cultured with either Teffs or Treg exp . Gene expression quantified by qRT-PCR comparing mRNA levels in M IL4 alone (RQ = 1, shown as dashed line) with the same cells co-cultured for 4h with either Teffs or Tregs. UBC was used as reference gene. (B) Intracellular concentration of cAMP in Tregs exp alone, M IL4 alone, or M IL4 co-cultured with Tregs exp for 4h. cAMP levels were normalized to total cellular protein content. Statistical analysis was performed only between M IL4 and M IL4 + Tregs exp , as the aim was to assess whether cAMP levels in M IL4 increase upon co-culture. The cAMP level in Tregs exp is shown as a reference to highlight the high concentration of this molecule in these cells but was not included in the statistical comparison due to the difference in cell type. (C) Changes in the expression of ABCA1 mRNA in M IL4 co-cultured with Treg exp (ratio 1:1) after 1h preincubation or not with GAP27 (300 µM). (D) Changes in the expression of ABCA1 mRNA in M IL4 co-cultured with Tregs (ratio 1:1) after 1h preincubation or not with PKA inhibitor H89 (5 µM). (E) Western blot analysis of ABCA1 protein level in cell lysates of M IL4 alone or M IL4 co-cultured (ratio 1:1) with either Treg exp or Teff for 4h. Data were plotted as ABCA1 protein intensity normalised to GAPDH protein intensity. Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparison test. *p<0.05, **p<0.01.

Article Snippet: Equal amounts of total protein were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to polyvinylidene difluoride (PVDF) membrane and probed overnight at 4C with the respective antibodies: monoclonal anti-ABCA1 antibody (CellSignaling; Cat Number 96292), polyclonal anti-PON1 antibody (Proteintech; Cat Number 18155-1-AP), monoclonal anti-GAPDH antibody (CellSignaling; Cat Number 5174), recombinant monoclonal anti-ABCA1 (phosho-S2054) antibody (Abcam; Cat Number ab125064).

Techniques: Expressing, Transferring, Cell Culture, Gene Expression, Quantitative RT-PCR, Concentration Assay, Co-Culture Assay, Comparison, Western Blot

Journal: Frontiers in Pharmacology

Article Title: Diterpenoids inhibit ox-LDL-induced foam cell formation in RAW264.7 cells by promoting ABCA1 mediated cholesterol efflux

doi: 10.3389/fphar.2023.1066758

Figure Lengend Snippet: Primers for real-time quantitative PCR.

Article Snippet: The rabbit monoclonal antibody against ABCA1 (Cat No. 96292S) was obtained from Cell Signaling Technology.

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Diterpenoids inhibit ox-LDL-induced foam cell formation in RAW264.7 cells by promoting ABCA1 mediated cholesterol efflux

doi: 10.3389/fphar.2023.1066758

Figure Lengend Snippet: Diterpenoids increase ABCA1 and ABCG1 expression. RAW264.7 macrophages were treated with 15 μmol/L indicated drugs and chemical compounds for 24 h. Cell lysates were collected, and the protein levels of PPARγ (A) and LXRα (B) were determined by western blotting. Corresponding quantitative results are shown as the mean ± SEM from three independent experiments (C,D) . Cellular RNA was collected for quantitative real-time PCR (qRT‒PCR) analysis. Data are shown as the mean ± SEM from three independent experiments (E,F) . Significance is presented as * p < .05 and ** p < .01 versus the vehicle (DMSO) control.

Article Snippet: The rabbit monoclonal antibody against ABCA1 (Cat No. 96292S) was obtained from Cell Signaling Technology.

Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction, Control

Journal: Frontiers in Pharmacology

Article Title: Diterpenoids inhibit ox-LDL-induced foam cell formation in RAW264.7 cells by promoting ABCA1 mediated cholesterol efflux

doi: 10.3389/fphar.2023.1066758

Figure Lengend Snippet: Treatment with diterpenoids increases the mRNA expression of ABCA1, LXRα, and PPARγ in ox-LDL-induced macrophage foam cells. RAW264.7 macrophages were treated with 15 μmol/L indicated compounds and 80 μg/mL ox-LDL for 24 h. Total RNA was extracted from the cells for quantitative real-time PCR (qRT‒PCR) analysis of the mRNA levels of ABCA1 (A) , ABCG1 (B) , LXRα (C) and PPARγ (D) . Data are shown as the mean ± SEM from three independent experiments. Significance is presented as * p < .05, ** p < .01 versus the vehicle (DMSO) group and # p < .05, ## p < .01 versus the control group.

Article Snippet: The rabbit monoclonal antibody against ABCA1 (Cat No. 96292S) was obtained from Cell Signaling Technology.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Control

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: MiR-145 is upregulated in the retarded preimplantation embryos and modulates cholesterol levels in mice preimplantation embryos through targeting Abca1

doi: 10.1186/s12958-022-01044-8

Figure Lengend Snippet: miR-145 directly targets Abca1. A Putative sequence complementarity between miR-145 and Abca1. B miR-145 mimic transfection was adequate to suppress the luciferase activity of a reporter containing the Abca1 3′-UTR segment harboring the binding site shown in (A) ( n = 3). C The overexpression of miR-145 in 293 T cells was confirmed via qPCR. D , E Abca1 mRNA and protein levels (cropped gels/blots) were assessed by means of qPCR and Western blotting after the indicated treatments. All the data are means ± s.d. from five independent experiments

Article Snippet: Blots were then proved overnight with monoclonal mouse anti-ABCA1 (Abcam, MA, USA; ab18180) or mouse anti-GAPDH (Abcam, MA, USA; ab8245) at 4 °C.

Techniques: Sequencing, Transfection, Luciferase, Activity Assay, Binding Assay, Over Expression, Western Blot

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: MiR-145 is upregulated in the retarded preimplantation embryos and modulates cholesterol levels in mice preimplantation embryos through targeting Abca1

doi: 10.1186/s12958-022-01044-8

Figure Lengend Snippet: miR-145 inhibits Abca1 expression and thereby increases cholesterol levels within embryos. A , B Abca1 expression at the mRNA level in blastocysts was assessed following overexpression of miR-145 in oocytes. Data are presented as means ± s.d. from five independent experiments (total number of blastocysts analyzed: n = 241 for control, 239 for miR-ctr, 226 for miR145 mimic and 229 for miR145 mu). B , C Representative western blot analysis for ABCA1 accumulation in blastocysts obtained from each groups (100 embryos per lane). One representative of three independent experiments is shown (B). Data are shown as medians with ranges. D miR-145 was associated with significantly increased cholesterol levels. Data are presented as means ± s.d. from five independent experiments (total number of n = 228 for control, n = 222 for miR-ctr, n = 212 for miR145 mimic and n = 218 for miR145 mu)

Article Snippet: Blots were then proved overnight with monoclonal mouse anti-ABCA1 (Abcam, MA, USA; ab18180) or mouse anti-GAPDH (Abcam, MA, USA; ab8245) at 4 °C.

Techniques: Expressing, Over Expression, Western Blot

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: MiR-145 is upregulated in the retarded preimplantation embryos and modulates cholesterol levels in mice preimplantation embryos through targeting Abca1

doi: 10.1186/s12958-022-01044-8

Figure Lengend Snippet: miR-145 regulates Abca1 expression to control murine embryonic development. A , B The overexpression of Abca1 was sufficient to reverse the retardation of embryonic growth observed in response to miR-145 overexpression. The images of embryos were taken at 74 h after transfer of the zygotes to KSOM. C Abca1 expression in the indicated groups was assessed via qPCR. Total number of zygotes used in (A)-(C), n = 180 for control, 121 for miR-ctr, 115 for miR145 mimic and 97 for miR145 + Abca1. Data are presented as means ± s.d. from five independent experiments. D Abca1 overexpression was sufficient to reverse miR-145 overexpression-induced increases in embryonic cholesterol levels. Total number of zygotes used in (D), n = 232 for control, 218 for miR-ctr, 209 for miR145 mimic and 195 for miR145 + Abca1. Data are presented as means ± s.d. from five independent experiments

Article Snippet: Blots were then proved overnight with monoclonal mouse anti-ABCA1 (Abcam, MA, USA; ab18180) or mouse anti-GAPDH (Abcam, MA, USA; ab8245) at 4 °C.

Techniques: Expressing, Over Expression

Journal: Nutrients

Article Title: Multifaceted Protective Effects of Hesperidin by Aromatic Hydrocarbon Receptor in Endothelial Cell Injury Induced by Benzo[a]Pyrene

doi: 10.3390/nu14030574

Figure Lengend Snippet: Effect of Hesperidin in mediating low density lipoprotein (LDL) accumulation in EA.hy926 induced by benzo[a]pyrene (BaP). ( A ) Representative immunocytochemistry of aromatic hydrocarbon receptor (AHR) level in EA.hy926 ( n = 3 per group, Scale bars, 100 μm). ( B ) mRNA levels of AHR in EA.hy926 ( n = 3 per group). * p < 0.05, vs. normal control group, # p < 0.05, vs. BaP control group. ( C ) mRNA levels of cytochrome P450 1A1 (CYP1A1) in EA.hy926 ( n = 3 per group). ** p < 0.01 vs. normal control group, ## p < 0.01 vs. BaP control group. ( D ) mRNA levels of CYP1A1 in EA.hy926 ( n = 3 per group). ** p < 0.01 vs. LDL control group, ## p < 0.01 vs. LDL + BaP group. ( E ) The uptake of FITC-LDL in EA.hy926 was observed with laser confocal microscopy ( n = 3 per group). ( F ) Representative immunocytochemistry of Adenosine Triphosphate-binding cassette transporter A1 (ABCA1) level in EA.hy926. ( n = 3 per group, Scale bars, 100 μm). ( G ) mRNA levels of ABC1A1 in EA.hy926 ( n = 3 per group) * p < 0.05, vs. normal control group, ## p < 0.01 vs. BaP control group. ( H ) Representative immunocytochemistry of advanced glycation end products (AGEs) level in EA.hy926. ( n = 3 per group, Scale bars, 100 μm). The data are presented as means ± SD ( n = 3) and were analyzed using one-way ANOVA. * p < 0.05, ** p < 0.01 vs. normal control group, # p < 0.05, ## p < 0.01 vs. BaP control group.

Article Snippet: For immunohistochemistry experiments, the cells were incubated with anti-AHR mouse monoclonal primary antibodies (1:200, SC133088, Santa Cruz, CA, USA), anti-ABCA1 mouse monoclonal primary antibodies (1:200, SC53482, Santa Cruz, CA, USA), anti-AGEs rabbit polyclonal primary antibodies (1:200, bs-1158R, BIOSS, Beijing, China), and anti-TNF-α rabbit polyclonal primary antibodies (1: 200, GTX110520, Gentex, MI, USA) overnight at 4 °C.

Techniques: Immunocytochemistry, Control, Confocal Microscopy, Binding Assay

Journal: Nutrients

Article Title: Multifaceted Protective Effects of Hesperidin by Aromatic Hydrocarbon Receptor in Endothelial Cell Injury Induced by Benzo[a]Pyrene

doi: 10.3390/nu14030574

Figure Lengend Snippet: Primers for real-time qPCR.

Article Snippet: For immunohistochemistry experiments, the cells were incubated with anti-AHR mouse monoclonal primary antibodies (1:200, SC133088, Santa Cruz, CA, USA), anti-ABCA1 mouse monoclonal primary antibodies (1:200, SC53482, Santa Cruz, CA, USA), anti-AGEs rabbit polyclonal primary antibodies (1:200, bs-1158R, BIOSS, Beijing, China), and anti-TNF-α rabbit polyclonal primary antibodies (1: 200, GTX110520, Gentex, MI, USA) overnight at 4 °C.

Techniques: Sequencing

Journal: Foods

Article Title: The Effects of Anthocyanin-Rich Bilberry Extract on Transintestinal Cholesterol Excretion

doi: 10.3390/foods10112852

Figure Lengend Snippet: The primer sequences for qRT-PCR analysis.

Article Snippet: Mouse monoclonal Anti-ABCA1 antibody , Abcam , ab18180.

Techniques:

Journal: Foods

Article Title: The Effects of Anthocyanin-Rich Bilberry Extract on Transintestinal Cholesterol Excretion

doi: 10.3390/foods10112852

Figure Lengend Snippet: Antibodies used for Western blot.

Article Snippet: Mouse monoclonal Anti-ABCA1 antibody , Abcam , ab18180.

Techniques: Western Blot